Math question related to science content

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Aug 17, 2023
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Hi im wondering if you can gelp i dont know how im supposed to calculate this.

We were given NaCl concentrations for 7 vials & one we didn't know the concentration of from a 1M per Litre stock solution. We added 250uL of each vials varying NaCl solutions to 500uL blood (so samples were each 750uL)

Our point was to apply them to blood samples, work out the hematocrit & graph it along with the osmolarities. From there we were told to estimate the osmolarity of vial 9 using the graph points (which I worked out vial 9 to be 0.4500 osmol/L). However a question is asking "what is the final NaCl in your unknown solution(sample 9).

I'm seriously confused how to work this out.

I'm not sure what other info is helpful. The concentrations of MOles per litre & osmolarity (osml/L) of the other vials were S1: 0.05M & 0.093. S2: 0.01M & 0.0186. S3: 0.154M & 0.2864. S4: 0.200M & 0.372. S5: 0.350M & 0.651. S6: 0.450M & 0.837. S7: 0.850M & 1.581 stock solution. We added 250uL of each vials solution to 500uL blood (so samples were each 750uL)

I was told to use the attached 3.1 equation which is attached but rearrange it, but that makes zero sense because I don't have the Moles in vial 9 solution
 

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